自然杂志 ›› 2018, Vol. 40 ›› Issue (2): 151-156.doi: 10.3969/j.issn.0253-9608.2018.02.010

• 科学人物 • 上一篇    

凝胶电泳和基因打靶——忆分子生物学家史密斯

郭晓强,桂耀庭   

  1. Shenzhen Hospital, Peking University, Shenzhen 518036, Guangdong Province, China
  • 收稿日期:2017-08-30 出版日期:2018-04-25 发布日期:2018-04-23
  • 作者简介:研究方向:肾癌发生机制的研究,主要涉及组蛋白修饰为主的表观遗传学、低氧信号通路与mTOR信号通路

A remembrance of molecular biologist Oliver Smithies: gel electrophoresis and gene targeting

GUO Xiaoqiang, GUI Yaoting   

  1. 北京大学深圳医院, 广东 深圳 518036
  • Received:2017-08-30 Online:2018-04-25 Published:2018-04-23

摘要:

史密斯是一位著名的分子生物学家,于20世纪50年代发明凝胶电泳,于80年代又发明基因打靶,从而为DNA、蛋白质体外分离和体内功能研究提供了重要的研究手段,极大地推动了生命科学的快速发展。史密斯还由于基因打靶的重要贡献而分享2007年诺贝尔生理学或医学奖。

关键词: 史密斯, 凝胶电泳, 基因打靶, 诺贝尔奖

Abstract:

Oliver Smithies is a famous molecular biologist, who invented gel electrophoresis in 1950s and gene targeting in 1980s. These methods provided important tools for the study of DNA and protein in vitro and in vivo, and greatly promoted the rapid development of life science. Oliver Smithies won the 2007 Nobel Prize in Physiology or Medicine for his groundbreaking contribution in invention of gene targeting technology. In the article, Oliver Smithies’ life, researches and achievements are introduced.