GUO Yusong R . Activation and regulation mechanism of Piezo channels as mechanosensors[J]. Chinese Journal of Nature, 2022 , 44(3) : 182 -194 . DOI: 10.3969/j.issn.0253-9608.2022.03.002

[1] JIN P, JAN L Y, JAN Y N. Mechanosensitive ion channels: structural features relevant to mechanotransduction mechanisms [J]. Annu Rev Neurosci, 2020, 43: 207-229.
[2] COSTE B, MATHUR J, SCHMIDT M, et al. Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels [J]. Science, 2010, 330(6000): 55-60.
[3] GE J, LI W, ZHAO Q, et al. Architecture of the mammalian mechanosensitive Piezo1 channel [J]. Nature, 2015, 527(7576): 64-69.
[4] GUO Y R, MACKINNON R. Structure-based membrane dome mechanism for Piezo mechanosensitivity [J]. eLife, 2017, 6:
e33660.
[5] SAOTOME K, MURTHY S E, KEFAUVER J M, et al. Structure of the mechanically activated ion channel Piezo1 [J]. Nature, 2018, 554(7693): 481-486.
[6] ZHAO Q, ZHOU H, CHI S, et al. Structure and mechanogating mechanism of the Piezo1 channel [J]. Nature, 2018, 554(7693): 487-492.
[7] GOTTLIEB P A, SACHS F. Piezo1: properties of a cation selective mechanical channel [J]. Channels, 2012, 6(4): 214-219.
[8] WU J, LEWIS A H, GRANDL J. Touch, tension, and transduction–the function and regulation of Piezo ion channels [J]. Trends in Biochemical Sciences, 2017, 42(1): 57-71.
[9] FOTIOU E, MARTIN-ALMEDINA S, SIMPSON M A, et al. Novel mutations in PIEZO1 cause an autosomal recessive
generalized lymphatic dysplasia with non-immune hydrops fetalis [J]. Nature Communications, 2015, 6: 8085.
[10] NONOMURA K, LUKACS V, SWEET D T, et al. Mechanically activated ion channel PIEZO1 is required for lymphatic valve
formation [J]. Proc Natl Acad Sci USA, 2018, 115(50): 12817-12822.
[11] KANG H, HONG Z, ZHONG M, et al. Piezo1 mediates angiogenesis through activation of MT1-MMP signaling [J]. Am J
Physiol Cell Physiol, 2019, 316(1): C92-C103.
[12] ANDOLFO I, ALPER S L, DE FRANCESCHI L, et al. Multiple clinical forms of dehydrated hereditary stomatocytosis arise from mutations in PIEZO1 [J]. Blood, 2013, 121(19): 3925-3935.
[13] CAHALAN S M, LUKACS V, RANADE S S, et al. Piezo1 links mechanical forces to red blood cell volume [J]. eLife, 2015, 4: e07370.
[14] MA S, CAHALAN S, LAMONTE G, et al. Common PIEZO1 allele in African populations causes RBC dehydration and
attenuates plasmodium infection [J]. Cell, 2018, 173(2): 443-455.
[15] KOSER D E, THOMPSON A J, FOSTER S K, et al. Mechanosensing is critical for axon growth in the developing brain
[J]. Nature Neuroscience, 2016, 19(12): 1592-1598.
[16] PATHAK M M, NOURSE J L, TRAN T, et al. Stretch-activated ion channel Piezo1 directs lineage choice in human neural stem cells [J]. Proceedings of the National Academy of Sciences, 2014, 111( 5): 16148-16153.
[17] FRIEDRICH E E, HONG Z, XIONG S, et al. Endothelial cell Piezo1 mediates pressure-induced eung vascular hyperpermeability via disruption of adherens junctions [J]. Proc Natl Acad Sci USA, 2019, 116(26): 12980-12985.

[18] PEYRONNET R, MARTINS J R, DUPRAT F, et al. Piezo1-dependent stretch-activated channels are inhibited by polycystin-2 in renal tubular epithelial cells [J]. EMBO Reports, 2013, 14(12): 1143-1148.

[19] DALGHI M G, CLAYTON D R, RUIZ W G, et al. Expression and distribution of PIEZO1 in the mouse urinary tract [J]. Am J Physiol Renal Physiol, 2019, 317(2): F303-F321.
[20] MARTINS J R, PENTON D, PEYRONNET R, et al. Piezo1-dependent regulation of urinary osmolarity [J]. Pflügers Archiv-
European Journal of Physiology, 2016, 468(7): 1197-1206.
[21] WANG L, ZHOU H, ZHANG M, et al. Structure and mechanogating of the mammalian tactile channel PIEZO2 [J].
Nature, 2019, 573(7773): 225-229.
[22] WU J, YOUNG M, LEWIS A H, et al. Inactivation of mechanically activated Piezo1 ion channels is determined by the C-terminal extracellular domain and the inner pore helix [J]. Cell Reports, 2017, 21(9): 2357-2366.
[23] SYEDA R, XU J, DUBIN A E, et al. Chemical activation of the mechanotransduction channel Piezo1 [J]. eLife, 2015, 4: e07369.
[24] RANADE S S, WOO S H, DUBIN A E, et al. Piezo2 is the major transducer of mechanical forces for touch sensation in mice [J]. Nature, 2014, 516(7529): 121-125.
[25] MAKSIMOVIC S, NAKATANI M, BABA Y, et al. Epidermal merkel cells are mechanosensory cells that tune mammalian touch receptors [J]. Nature, 2014, 509(7502): 617-621.
[26] WOO S H, RANADE S, WEYER A D, et al. Piezo2 is required for merkel-cell mechanotransduction [J]. Nature, 2014, 509(7502): 622-626.
[27] WOO S H, LUKACS V, DE NOOIJ J C, et al. Piezo2 is the principal mechanotransduction channel for proprioception [J].
Nature Neuroscience, 2015, 18(12): 1756-1762.
[28] MAHMUD A A, NAHID N A, NASSIF C, et al. Loss of the proprioception and touch sensation channel PIEZO2 in siblings
with a progressive form of contractures [J]. Clin Genet, 2017, 91(3): 470-475.
[29] MURTHY S E, LOUD M C, DAOU I, et al. The mechanosensitive ion channel Piezo2 mediates sensitivity to mechanical pain in mice [J]. Sci Transl Med, 2018, 10(462): eaat9897.
[30] SZCZOT M, LILJENCRANTZ J, GHITANI N, et al. PIEZO2 mediates injury-induced tactile pain in mice and humans [J]. Sci Transl Med, 2018, 10(462): eaat9892.
[31] MIKHAILOV N, LESKINEN J, FAGERLUND I, et al. Mechanosensitive meningeal nociception via Piezo channels: implications for pulsatile pain in migraine? [J]. Neuropharmacology, 2019, 149: 113-123.
[32] ZENG W Z, MARSHALL K L, MIN S, et al. PIEZOs mediate neuronal sensing of blood pressure and the baroreceptor reflex [J]. Science, 2018, 362(6413): 464-467.
[33] LEE W, LEDDY H A, CHEN Y, et al. Synergy between Piezo1 and Piezo2 channels confers high-strain mechanosensitivity to articular cartilage [J]. Proceedings of the National Academy of Sciences, 2014, 111(47): E5114-E5122.
[34] OKUBO M, FUJITA A, SAITO Y, et al. A family of distal arthrogryposis type 5 due to a novel PIEZO2 mutation [J].
American Journal of Medical Genetics Part A, 2015, 167(5): 1100-1106.
[35] COSTE B, HOUGE G, MURRAY M F, et al. Gain-of-function mutations in the mechanically activated ion channel PIEZO2 cause a subtype of distal arthrogryposis [J]. Proceedings of the National Academy of Sciences, 2013, 110(12): 4667-4672.
[36] WANG F, KNUTSON K, ALCAINO C, et al. Mechanosensitive ion channel Piezo2 is important for enterochromaffin cell response to mechanical forces: Piezo2 in EC cells [J]. The Journal of Physiology, 2017, 595(1): 79-91.
[37] MAZZUOLI-WEBER G, KUGLER E M, BÜHLER C I, et al. Piezo proteins: incidence and abundance in the enteric nervous
system. Is there a link with mechanosensitivity? [J]. Cell Tissue Res, 2019, 375(3): 605-618.
[38] ALBUISSON J, MURTHY S E, BANDELL M, et al. Dehydrated hereditary stomatocytosis linked to gain-of-function mutations in mechanically activated PIEZO1 ion channels [J]. Nature Communications, 2013, 4. DOI: 10.1038/ncomms2899.
[39] MCMILLIN M J, BECK A E, CHONG J X, et al. Mutations in PIEZO2 cause gordon syndrome, marden-walker syndrome, and distal arthrogryposis Type 5 [J]. Am J Hum Genet, 2014, 94(5): 734-744.
[40] RETAILLEAU K, DUPRAT F, ARHATTE M, et al. Piezo1 in smooth muscle cells is involved in hypertension-dependent arterial remodeling [J]. Cell Reports, 2015, 13(6): 1161-1171.
[41] DELLA PIETRA A, MIKHAILOV N, GINIATULLIN R. The emerging role of mechanosensitive Piezo channels in migraine pain[J]. Int J Mol Sci, 2020, 21(3): E696.
[42] SUN Y, LI M, LIU G, et al. The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism [J]. J Cancer Res Clin Oncol, 2020, 146(5): 1139-1152.
[43] HAN Y, LIU C, ZHANG D, et al. Mechanosensitive ion channel Piezo1 promotes prostate cancer development through the activation of the akt/MTOR pathway and acceleration of cell cycle [J]. Int J Oncol, 2019, 55(3): 629-644.
[44] AYKUT B, CHEN R, KIM J I, et al. Targeting Piezo1 unleashes innate immunity against cancer and infectious disease [J]. Sci Immunol, 2020, 5(50): eabb5168.
[45] YANG H, LIU C, ZHOU R M, et al. Piezo2 protein: a novel regulator of tumor angiogenesis and hyperpermeability [J].
Oncotarget, 2016, 7(28): 44630.
[46] SUKHAREV S I, BLOUNT P, MARTINAC B, et al. Mechanosensitive channels of escherichia coli: The MscL gene,
protein, and activities [J]. Annual Review of Physiology, 1997, 59(1): 633-657.
[47] ISCLA I, BLOUNT P. Sensing and responding to membrane tension: the bacterial MscL channel as a model system [J].
Biophysical Journal, 2012, 103(2): 169-174.
[48] PEROZO E, CORTES D M, SOMPORNPISUT P, et al. Open channel structure of MscL and the gating mechanism of
mechanosensitive channels [J]. Nature, 2002, 418(6901): 942-948.
[49] CHANG G, SPENCER R H, LEE A T, et al. Structure of the MscL homolog from mycobacterium tuberculosis: a gated
mechanosensitive ion channel [J]. Science, 1998, 282(5397): 2220-2226.
[50] SUKHAREV S, DURELL S R, GUY H R. Structural models of the MscL gating mechanism [J]. Biophysical Journal, 2001, 81(2): 917-936.
[51] CORRY B, HURST A C, PAL P, et al. An improved open-channel structure of MscL determined from FRET confocal microscopy and simulation [J]. The Journal of General Physiology, 2010, 136(4): 483-494.
[52] WIGGINS P, PHILLIPS R. Membrane-protein interactions in mechanosensitive channels [J]. Biophysical Journal, 2005, 88(2): 880-902.
[53] HASWELL E S, PHILLIPS R, REES D C. Mechanosensitive channels: What can they do and how do they do it? [J]. Structure, 2011, 19(10): 1356-1369.
[54] MARTINAC B, ADLER J, KUNG C. Mechanosensitive ion channels of E. coli activated by amphipaths [J]. Nature, 1990,
348(6298): 261-263.
[55] BROHAWN S G, SU Z, MACKINNON R. Mechanosensitivity is mediated directly by the lipid membrane in TRAAK and TREK1 K⁺ channels [J]. Proceedings of the National Academy of Sciences, 2014, 111(9): 3614-3619.
[56] BROHAWN S G, CAMPBELL E B, MACKINNON R. Physical mechanism for gating and mechanosensitivity of the human
TRAAK K + channel [J]. Nature, 2014, 516(7529): 126-130.
[57] JIAO R, CUI D, WANG S C, et al. Interactions of the mechanosensitive channels with extracellular matrix, integrins, and
cytoskeletal network in osmosensation [J]. Front Mol Neurosci, 2017, 10: 96.
[58] HOLT J R, TOBIN M, ELFERICH J, et al. Putting the pieces together: the hair cell transduction complex [J]. J Assoc Res
Otolaryngol, 2021, 22(6): 601-608.
[59] JIN P, BULKLEY D, GUO Y, et al. Electron cryo-microscopy structure of the mechanotransduction channel NOMPC [J]. Nature, 2017, 547(7661): 118-122.
[60] SUN L, GAO Y, HE J, et al. Ultrastructural organization of NompC in the mechanoreceptive organelle of drosophila campaniform mechanoreceptors [J]. Proc Natl Acad Sci USA, 2019, 116(15): 7343-7352.
[61] DOYLE D A, MORAIS CABRAL J, PFUETZNER R A, et al. The structure of the potassium channel: Molecular basis of K +
conduction and selectivity [J]. Science, 1998, 280(5360): 69-77.
[62] SYEDA R, FLORENDO M N, COX C D, et al. Piezo1 channels are inherently echanosensitive [J]. Cell Reports, 2016, 17(7): 1739-1746.
[63] POOLE K, HERGET R, LAPATSINA L, et al. Tuning Piezo ion channels to detect molecular-scale movements relevant for fine touch [J]. Nature Communications, 2014, 5: 3520.
[64] WU J, GOYAL R, GRANDL J. Localized force application reveals mechanically sensitive domains of Piezo1 [J]. Nature
Communications, 2016, 7: 12939.
[65] GAO Q, COOPER P R, WALMSLEY A D, et al. Role of Piezo channels in ultrasound-stimulated dental stem cells [J]. Journal of Endodontics, 2017, 43(7): 1130-1136.
[66] PRIETO M L, FIROUZI K, KHURI-YAKUB B T, et al. Activation of Piezo1 but not NaV1.2 channels by ultrasound at 43 MHz [J]. Ultrasound Med Biol, 2018, 44(6): 1217-1232.
[67] GAUB B M, MÜLLER D J. Mechanical stimulation of Piezo1 receptors depends on extracellular matrix proteins and directionality of force [J]. Nano Letters, 2017, 17(3): 2064-2072.
[68] LIN Y C, GUO Y R, MIYAGI A, et al. Force-induced conformational changes in PIEZO1 [J]. Nature, 2019, 573(7773):
230-234.
[69] WANG Y, CHI S, GUO H, et al. A lever-like transduction pathway for long-distance chemical- and mechano-gating of the
mechanosensitive Piezo1 channel [J]. Nature Communications, 2018, 9(1): 1300.
[70] LACROIX J J, BOTELLO-SMITH W M, LUO Y. Probing the gating mechanism of the mechanosensitive channel Piezo1 with the small molecule Yoda1 [J]. Nature Communications, 2018, 9(1): 2029.
[71] ROTORDAM M G, FERMO E, BECKER N, et al. A yoda1-based approach to investigate Piezo1 channels in red blood cells using automated patch clamp technology [J]. Blood, 2018, 132: 1031.
[72] LIN Y, BUYAN A, CORRY B. Computational studies of Piezo1 yield insights into key lipid–protein interactions, channel activation, and agonist binding [J]. Biophys Rev, 2022, 14: 209-219. 

[73] SUKHAREV S I, MARTINAC B, ARSHAVSKY V Y, et al. Two types of mechanosensitive channels in the escherichia coli cell envelope: solubilization and functional reconstitution [J]. Biophysical Journal, 1993, 65(1): 177-183.
[74] LEWIS A H, GRANDL J. Inactivation kinetics and mechanical gating of Piezo1 ion channels depend on subdomains within the cap [J]. Cell Reports, 2020, 30(3): 870-880.
[75] JIANG W, DEL ROSARIO J S, BOTELLO-SMITH W, et al. Crowding-induced opening of the mechanosensitive Piezo1 channel in silico [J]. Communications Biology, 2021, 4(1): 1-14.
[76] VECCHIS D D, BEECH D J, KALLI A C. Molecular dynamics simulations of Piezo1 channel opening by increases in membrane tension [J]. Biophysical Journal, 2021, 120(8): 1510-1521.
[77] YANG X, LIN C, CHEN X, et al. Structure deformation and curvature sensing of PIEZO1 in lipid membranes [J]. Nature, 2022, 604(7905): 377-383.
[78] HASELWANDTER C A, MACKINNON R. Piezo’s membrane footprint and its contribution to mechanosensitivity [J]. eLife, 2018, 7: e41968.
[79] GENG J, LIU W, ZHOU H, et al. A plug-and-latch mechanism for gating the mechanosensitive Piezo channel [J]. Neuron, 2020, 106(3): 438-451.
[80] ZHAO Q, WU K, GENG J, et al. on Permeation and mechanotransduction mechanisms of mechanosensitive Piezo channels [J]. Neuron, 2016, 89(6): 1248-1263.
[81] BROHAWN S G, WANG W, HANDLER A, et al. The mechanosensitive ion channel TRAAK is localized to the
mammalian node of ranvier [J]. eLife, 2019, 8: e50403.
[82] JIA Y, ZHAO Y, KUSAKIZAKO T, et al. TMC1 and TMC2 proteins are pore-forming subunits of mechanosensitive ion
channels [J]. Neuron, 2020, 105(2): 310-321.
[83] YUE X, SHENG Y, KANG L, et al. Distinct functions of TMC channels: a comparative overview [J]. Cell Mol Life Sci, 2019,
76(21): 4221-4232.
[84] WALKER R G, WILLINGHAM A T, ZUKER C S. A Drosophila mechanosensory transduction channel [J]. Science, 2000,
287(5461): 2229-2234.
[85] YAN Z, ZHANG W, HE Y, et al. Drosophila NOMPC is a mechanotransduction channel subunit for gentle-touch sensation
[J]. Nature, 2013, 493(7431): 221-225.
[86] KANG L, GAO J, SCHAFER W R, et al. Elegans TRP family protein TRP-4 is a pore-forming subunit of a native
mechanotransduction channel [J]. Neuron, 2010, 67(3): 381-391.
[87] MURTHY S E, DUBIN A E, WHITWAM T, et al. OSCA/TMEM63 are an evolutionarily conserved family of mechanically activated ion channels [J]. eLife, 2018, 7: e41844.
[88] BEAULIEU-LAROCHE L, CHRISTIN M, DONOGHUE A, et al. TACAN is an ion channel involved in sensing mechanical pain [J]. Cell, 2020, 180(5): 956-967.
[89] CHEN X, WANG Y, LI Y, et al. Cryo-EM structure of the human TACAN in a closed state [J]. Cell Reports, 2022, 38(9): 110445.
[90] NIU Y, TAO X, VAISEY G, et al. Analysis of the mechanosensor channel functionality of TACAN [J]. eLife, 2021, 10: e71188.
[91] RONG Y, JIANG J, GAO Y, et al. TMEM120A contains a specific coenzyme A-binding site and might not mediate poking- or stretch-induced channel activities in cells [J]. eLife, 2021, 10: e71474.
[92] XUE J, HAN Y, BANIASADI H, et al. TMEM120A is a coenzyme A-binding membrane protein with structural similarities to ELOVL fatty acid elongase [J]. eLife, 2021, 10: e71220.
[93] FANG X Z, ZHOU T, XU J Q, et al. Structure, kinetic properties and biological function of mechanosensitive Piezo channels [J]. Cell & Bioscience, 2021, 11(1): 13.
[94] DEL MÁRMOL J I, TOUHARA K K, CROFT G, et al. Piezo1 forms a slowly-inactivating mechanosensory channel in mouse
embryonic stem cells [J]. eLife, 2018, 7: e33149.
[95] QI Y, ANDOLFI L, FRATTINI F, et al. Membrane stiffening by STOML3 facilitates mechanosensation in sensory neurons [J]. Nature Communications, 2015, 6: 8512.
[96] ZHANG T, CHI S, JIANG F, et al. A protein interaction mechanism for suppressing the mechanosensitive Piezo channels [J]. Nature Communications, 2017, 8(1): 1797.
[97] ALPER S L. Genetic diseases of PIEZO1 and PIEZO2 dysfunction [J]. In Current Topics in Membranes, 2017, 79: 97-134.
[98] SZCZOT M, POGORZALA L A, SOLINSKI H J, et al. Cell-type-specific splicing of Piezo2 regulates mechanotransduction [J]. Cell Reports, 2017, 21 (10): 2760-2771.
[99] VERKEST C, SCHAEFER I, NEES T A, et al. Intrinsically disordered intracellular domains control key features of
the mechanically-gated ion channel PIEZO2 [J]. Nature Communications, 2022, 13 (1): 1365.
[100] RIDONE P, PANDZIC E, VASSALLI M, et al. Disruption of membrane cholesterol organization impairs the activity of PIEZO1 channel clusters [J]. Journal of General Physiology, 2020, 152: e201912515.
[101] LEWIS A H, GRANDL J. Piezo1 ion channels inherently function as independent mechanotransducers [J]. eLife, 2021, 10: e70988.
[102] ISCLA I, WRAY R, EATON C, et al. Scanning MscL channels with targeted post-translational modifications for functional alterations [J]. PLoS One, 2015, 10(9): e0137994.
[103] SHIPSTON M J. Ion channel regulation by protein S-acylation [J]. Journal of General Physiology, 2014, 143(6): 659-678.
[104] LI J V, NG C A, CHENG D, et al. Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutations [J]. Commun Biol, 2021, 4(1): 1-17.
[105] COSTE B, XIAO B, SANTOS J S, et al. Piezo proteins are pore-forming subunits of mechanically activated channels [J]. Nature,2012, 483(7388): 176-181.
[106] ROMERO L O, MASSEY A E, MATA-DABOIN A D, et al. Dietary fatty acids fine-tune Piezo1 mechanical response [J].
Nature Communications, 2019, 10 (1): 1200.
[107] BORBIRO I, BADHEKA D, ROHACS T. Activation of TRPV1 channels inhibits mechanosensitive Piezo channel activity by
depleting membrane phosphoinositides [J]. Science Signaling, 2015, 8(363): ra15.
[108] NARAYANAN P, HÜTTE M, KUDRYASHEVA G, et al. Myotubularin related protein-2 and its phospholipid substrate
PIP2 control Piezo2-mediated mechanotransduction in peripheral sensory neurons [J]. eLife, 2018, 7: e32346.
[109] SHI J, HYMAN A J, DE VECCHIS D, et al. Sphingomyelinase disables inactivation in endogenous PIEZO1 channels [J]. Cell Reports, 2020, 33(1): 108225.
[110] GNANASAMBANDAM R, GHATAK C, YASMANN A, et al. GsMTx4: mechanism of inhibiting mechanosensitive ion channels [J]. Biophysical Journal, 2017, 112(1): 31-45.
[111] MANESHI M M, ZIEGLER L, SACHS F, et al. Enantiomeric Aβ peptides inhibit the fluid shear stress response of PIEZO1 [J]. Scientific Reports, 2018, 8 (1): 14267.
[112] CHONG J, DE VECCHIS D, HYMAN A J, et al. Modeling of full-length Piezo1 suggests importance of the proximal N-terminus fordome structure [J]. Biophysical Journal, 2021, 120(8): 1343-1356.
[113] BUYAN A, COX C D, BARNOUD J, et al. Piezo1 forms specific, functionally important interactions with phosphoinositides and cholesterol [J]. Biophysical Journal, 2020, 119(8): 1683-1697.
[114] NARAYANAN P, SONDERMANN J, ROUWETTE T, et al. Native Piezo2 interactomics identifies pericentrin as a novel
regulator of Piezo2 in somatosensory neurons [J]. Journal of Proteome Research, 2016, 15(8): 2676-2687.
[115] GOTTLIEB P A, BAE C, SACHS F. Gating the mechanical channel Piezo1: a comparison between whole-cell and patch
recording [J]. Channels, 2012, 6(4): 282-289.
[116] COX C D, BAE C, ZIEGLER L, et al. Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1
is gated by bilayer tension [J]. Nature Communications, 2016, 7: 10366.
[117] ELLEFSEN K L, HOLT J R, CHANG A C, et al. Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca ²⁺ flickers [J]. Commun Biol, 2019, 2(1): 1-13.
[118] WANG J, JIANG J, YANG X, et al. Tethering Piezo channels to the actin cytoskeleton for mechanogating via the E-cadherin-β-catenin mechanotransduction complex [J]. Cell Reports, 2020, 38(6). DOI: 10.1101/2020.05.12.092148.
[119] DEL ROSARIO J S, YUDIN Y, SU S, et al. Gi-coupled receptor activation potentiates Piezo2 currents via Gβγ [J]. EMBO Rep, 2020, 21(5): e49124.
[120] EVANS E L, CUTHBERTSON K, ENDESH N, et al. Yoda1 analogue (Dooku1) which antagonizes yoda1-evoked activation of
Piezo1 and aortic relaxation [J]. Br J Pharmacol, 2018, 175(10):
1744-1759.
[121] LIU S, PAN X, CHENG W, et al. Tubeimoside I antagonizes
Yoda1-evoked Piezo1 channel activation [J]. Frontiers in
Pharmacology, 2020, 11: 768.
[122] BOTELLO-SMITH W M, JIANG W, ZHANG H, et al. A
mechanism for the activation of the mechanosensitive Piezo1
channel by the small molecule Yoda1 [J]. Nature Communications,
2019, 10(1): 4503.
[123] LI H, WEI W, XU H. Drug discovery is an eternal challenge for
the biomedical sciences [J]. Acta Materia Medica, 2022. DOI:
10.15212/AMM-2022-1001.
[124] ZHANG M, WANG D, KANG Y, et al. Structure of the
mechanosensitive OSCA channels [J]. Nat Struct Mol Biol, 2018,
25(9): 850-858.
[125] GE J, ELFERICH J, GOEHRING A, et al. Structure of mouse
protocadherin 15 of the stereocilia tip link in complex with LHFPL5 [J]. eLife, 2018, 7: e38770.
[126] NORENG S, BHARADWAJ A, POSERT R, et al. Structure of the human epithelial sodium channel by cryo-electron microscopy [J]. eLife, 2018, 7: e39340.
[127] MAITY K, HEUMANN J M, MCGRATH A P, et al. Cryo-EM structure of OSCA1.2 from oryza sativa elucidates the mechanical basis of potential membrane hyperosmolality gating [J]. Proceedings of the National Academy of Sciences, 2019, 116(28): 14309-14318.
[128] ZHANG Y, DADAY C, GU R X, et al. Visualization of the mechanosensitive ion channel MscS under membrane tension [J]. Nature, 2021, 590(7846): 509-514.
[129] BAE C, SACHS F, GOTTLIEB P A. Protonation of the human PIEZO1 ion channel stabilizes inactivation [J]. Journal of
Biological Chemistry, 2015, 290(8): 5167-5173.
[130] BAE C, GOTTLIEB P A, SACHS F. Human PIEZO1: removing inactivation [J]. Biophysical Journal, 2013, 105(4): 880-886.
[131] ZHENG W, GRACHEVA E O, BAGRIANTSEV S N. A hydrophobic gate in the inner pore helix is the major determinant
of inactivation in mechanosensitive Piezo channels [J]. eLife, 2019, 8: e44003.
[132] EVANS E L, POVSTYAN O V, DE VECCHIS D, et al. RBCs prevent rapid PIEZO1 inactivation and expose slow deactivation as a mechanism of dehydrated hereditary stomatocytosis [J]. Blood, 2020, 136(1): 140-144.
[133] HENDRICKX G, FISCHER V, LIEDERT A, et al. Piezo1 inactivation in chondrocytes impairs trabecular bone formation [J]. Journal of Bone and Mineral Research, 2021, 36(2): 369-384.
[134] DEMOLOMBE S, DUPRAT F, HONORÉ E, et al. Slower Piezo1 inactivation in dehydrated hereditary stomatocytosis (xerocytosis) [J]. Biophysical Journal, 2013, 105(4): 833-834.