Abstract: The delivery of exogenous macromolecules into cells is very important in both basic biology and clinical applications, but the traditional biological methods using viral vectors have problems such as low throughput, high toxicity, low delivery performance and time consuming. In this review, it is summarized that electroporation, sonoporation, microinjection, cell extrusion and other technologies to achieve intracellular delivery based on cell membrane destruction. These physical methods of high throughput intracellular delivery through micro and nano technology can avoid many defects of traditional biological methods, and experiments have proved that they can achieve considerable delivery efficiency while guaranteeing cell activity. We focus on recent development emergence of microfluidicplatform based cell extrusion for gene delivery and their mechanisms, and discuss the advantages, limitations and development prospects of these technologies for delivering cellular drugs or molecules through cell membrane destruction.