Abstract: When the audience enjoy painting, his feeling is a complex process of physiology, psychology and other aspects.
Scientists have studied it for many years, and obtained some remarkable results. The author introduces the influence of visual
physiological characteristics of Mach band effect, eyeball movement and Purkinje phenomenon on painting appreciation.

Key words:  Mona Lisa’s smile, eyeball movement, Purkinje phenomenon, spatial frequency, Mach band effect