Chinese Journal of Nature ›› 2021, Vol. 43 ›› Issue (5): 374-382.doi: 10.3969/j.issn.0253-9608.2021.05.008

• Special Issue for Advanced Immunochemical Studies • Previous Articles     Next Articles

Advances in combinatorial antibody library

QIANG Min, SHI Xiaojie, ZHANG Chuyue, MA Peixiang, YANG Guang   

  1. Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China
  • Received:2021-05-30 Online:2021-10-22 Published:2021-10-25

Abstract: Combinatorial antibody library is a key strategy in immunochemistry, which aims to establish libraries of large capacity and high diversity in vitro. It integrates the genotype and phenotype in the screening system to allow the molecule to self-replicate. It leverages the principle of biological evolution to perform high-throughput screening. This technology allows the reconstruction of an immune system in a test tube and empowers the antibody selection without the constraints of animals or organs. It records the donors’ immune response by molecular clone. The random recombination of heavy and light chains can avoid immunologic tolerance and discover rare antibodies. Due to the development of immunochemistry, the combinatorial antibody library technology keeps updating. Novel screening approaches have emerged, such as autocrine cell-based selection system and cell-cell interaction based system. Here, we introduce the development of combinatorial antibody library technology.