Abstract: The migration routes of Great Black-headed Gull and Bar-headed Gull breeding at Qinghai Lake were studied using satellite-tracking from July of 2006 to July of 2007. Four Great Black-headed Gulls and one Bar-headed Goose banded with satellite transmitters, arrived at their wintering sites and backed to Qinghai Lake in next year successfully. In this paper, the migration routes of Great Black-headed Gull and Bar-headed Goose were clarified, combining the application of satellite-tracking on birds migration, which provides the scientific evidences on the relationship between Avian Influenza and bird migration at Qinghai Lake, even at the Central Asia-India flyway.