关键词: 皮肤类器官, 皮肤附属物类器官, 类器官构建, 类器官应用, 疾病模型, 药效评价

Abstract:  Skin, the largest organ of the human body, is composed of closely adjacent epidermis and dermis, which plays vital roles in human life, including barrier function, thermoregulation, sensation, and immunity. In recent years, with the rapid advancement of organoid research, skin organoids have garnered increasing attentions and have been widely applied. This review systematically summarizes current methodologies for constructing both composite skin organoids and individual skin appendage organoids, encompassing differentiation induction from induced pluripotent stem cells (iPSCs) to self-organization of adult stem cells into organoids. Additionally, it outlines the diverse applications of skin organoids, such as disease modeling, regeneration of tissues and organs, tumor research, and drug efficacy evaluation. In addition to comprehensively evaluating existing constraints in skin organoid development, we present targeted recommendations for technological innovation, establishing a conceptual foundation for advancing this field.