自然杂志 ›› 2021, Vol. 43 ›› Issue (5): 359-364.doi: 10.3969/j.issn.0253-9608.2021.05.006

• 免疫化学专刊 • 上一篇    下一篇

C2H2锌指蛋白药物递送技术的研发与应用

李璐,魏旋,刘佳   

  1. 上海科技大学 免疫化学研究所,上海 201210
  • 收稿日期:2021-06-19 出版日期:2021-10-22 发布日期:2021-10-22
  • 通讯作者: 刘佳,通信作者,研究方向:大分子药物递呈。

Development and application of C2H2 zinc finger protein-based drug delivery technology

LI Lu, WEI Xuan, LIU Jia   

  1. Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China
  • Received:2021-06-19 Online:2021-10-22 Published:2021-10-22

摘要: 锌指蛋白是真核生物中广泛存在的转录因子,在基因的表达调控中起着重要作用。近期研究发现,C2H2类型的锌指蛋白(通过两个半胱氨酸和两个组氨酸螯合锌离子)可通过巨胞饮进入哺乳动物细胞。进一步的研究发现C2H2锌指蛋白可介导多种模式蛋白质及药物蛋白质的高效细胞递送,其细胞穿透效率亦高于传统的细胞穿膜肽。文章介绍了锌指蛋白的基本生物学信息和其细胞穿透特性,并讨论C2H2 锌指蛋白在大分子药物递送中的应用。

关键词: 锌指蛋白, 药物递送, 内化机制, 临床应用

Abstract: Zinc finger proteins (ZFPs) are widespread transcription factors in eukaryotes and play important roles in gene expression
regulation. Recent studies have shown that C2H2 ZFPs can penetrate mammalian cells through macropinocytosis. In further studies, we have found that C2H2 ZFPs are capable of achieving highly efficient cellular delivery of a wide range of proteins including both reporter proteins and therapeutic proteins, with superior cell-penetrating activity to traditional cell-penetrating peptides (CPPs). In this review, we introduce the basic biology and the cell-penetrating activities of ZFPs, and discuss the application of C2H2 ZFPs in macromolecular drug delivery.