关键词: 组蛋白去乙酰化酶, Clr6S, Rpd3S, 核小体, 冷冻电镜

Abstract: Type I histone deacetylase complex (type I HDAC complex), conserved from yeast to human, is a zinc-dependent histone modifying enzyme. Both Saccharomyces cerevisiae Rpd3S and Schizosaccharomyces pombe Clr6S, two homologous multiplesubunit complexes, are recruited by histone H3 methylation K36 (H3K36me) to open reading frame and remove the acetyl groups from specific lysine residues at histones H3 and H4, thereby suppressing cryptic transcription. In this review, the recent cryo-EM work on type I HDAC complexes is summarized, and the mechanisms of nucleosome recognition and subsequent deacetylation by type I HDAC complex are discussed.