蛋白质剪接在蛋白质研究和蛋白质工程中的应用

自然杂志 ›› 2012, Vol. 34 ›› Issue (1): 32-38.

蛋白质剪接在蛋白质研究和蛋白质工程中的应用

戴旭东①孟清②刘相钦③

①博士研究生，②教授，东华大学生物科学与技术研究所,上海201620; ③教授，Department of Biochemistry & Molecular Biology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H1X5

收稿日期:2011-09-30 修回日期:2011-10-30 出版日期:2012-02-25 发布日期:2012-02-25

Protein Splicing Technology in Protein Research and Protein Engineering

DAI Xu-dong①, MENG Qing②, LIU Xiang-qin③

①Ph. D. Candidate， ②Professor, Institute of Biological Science and Technology, Donghua University, Shanghai 201620, China; ③Professor，Department of Biochemistry & Molecular Biology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H1X5

Received:2011-09-30 Revised:2011-10-30 Online:2012-02-25 Published:2012-02-25

摘要/Abstract

摘要： 蛋白质剪接(protein splicing)是由蛋白质内含子介导的在蛋白质水平上翻译后的加工过程。蛋白质内含子(intein)是指前体蛋白中的一段插入序列，它在蛋白质翻译后的成熟过程中能自我催化，使自身从前体蛋白中切除，并将其两侧的多肽片段以肽键连接，形成成熟的蛋白质。蛋白质内含子的发现丰富了基因表达和蛋白质翻译成熟过程的理论，而且在蛋白质研究和蛋白质工程中有广泛的应用。本文试图综述蛋白质剪接在蛋白质特异位点标记、蛋白质片段化标记同位素、蛋白质环化、蛋白质芯片、基因治疗等研究中的应用。

关键词: 蛋白质剪接, 断裂蛋白质内含子, 蛋白质研究, 蛋白质工程

Abstract: Protein splicing is posttranslational process mediated by inteins. Inteins are internal protein sequences that selfexcise and splice together the flanking sequences with a peptide bond. The discovery of inteins enhances theories of gene expression and posttranslation, protein splicing technology can be applied in protein research and protein engineering. This review provides a survey of recent applications of protein splicing including protein labeling, protein segmental isotopelabeling, protein cyclization, protein chip, gene therapy and so on.

Key words: protein splicing, split protein, protein research, protein engineering

戴旭东孟清刘相钦. 蛋白质剪接在蛋白质研究和蛋白质工程中的应用[J]. 自然杂志, 2012, 34(1): 32-38.

DAI Xu-Dong;MENG Qing;LIU Xiang-Qin. Protein Splicing Technology in Protein Research and Protein Engineering[J]. Chinese Journal of Nature, 2012, 34(1): 32-38.