Abstract: In the last half of 20th century, molecular biology has made rapid development in life science. The discovery and application of molecular biology enzymes play an important role in promoting it. Several enzymes were identified and clarified including DNA polymerase, RNA polymerase, reverse transcriptase, restriction endonuclease, and telomerase. The progresses expand the understanding of many life phenomena. These enzymes also were used in molecular biology tecniques, known as recombinant DNA, Sanger sequencing and polymerase chain reaction, which have a variety of applications such as nucleic acid manipulation, DNA sequencing and amplification. In the paper, the history of researches on molecular biology enzymes was described to understand the
significance of these enzymes.