DNA聚合酶Ⅲ全酶的功能和结构的发现

自然杂志 ›› 2012, Vol. 34 ›› Issue (4): 231-240.

DNA聚合酶Ⅲ全酶的功能和结构的发现

向义和

教授，清华大学物理系，北京 100084

收稿日期:2012-01-03 修回日期:2012-02-03 出版日期:2012-08-25 发布日期:2012-08-25

Discovery of Function and Structure of Deoxyribonucleic Acid Polymerase Ⅲ Holoenzyme

XIANG Yi-he

Professor, Department of Physics, Tsinghua University, Beijing 100084, China

Received:2012-01-03 Revised:2012-02-03 Online:2012-08-25 Published:2012-08-25

摘要/Abstract

摘要： 介绍了DNA聚合酶Ⅲ和聚合酶Ⅲ*的发现，DNA聚合酶Ⅲ全酶形式的提出以及全酶亚基的分离。同时，介绍了DNA聚合酶Ⅲ全酶的结构和功能，其中包括α核心的聚合酶功能，ε亚基的3′→5′外切核酸酶活性，[WTHZ]β[WTH1]亚基滑动夹子的功能以及γ复合物的结构与功能。

关键词: DNA聚合酶Ⅲ, DNA聚合酶Ⅲ*, 共聚物酶Ⅲ*, α核心聚合酶, ε亚基, β亚基滑动夹子, γ复合物

Abstract: The process of finding the structure and the function of DNA polymerase Ⅲ holoenzyme are introduced. The key events include that the discovery of DNA polymerase Ⅲ and Ⅲ* , the proposition of DNA polymerase Ⅲ holoenzyme, the separation of holoenzyme subunit, the function of core polymerase, the 3→5 exonuclease activity of ε subunit，the function of β subunit sliding clamp and γ complex.[JP]

Key words: DNA polymerase Ⅲ, DNA polymeraseⅢ*, Copolymerase Ⅲ*, core polymerase, ε subunit, β subunit sliding clamp, γ complex

向义和. DNA聚合酶Ⅲ全酶的功能和结构的发现[J]. 自然杂志, 2012, 34(4): 231-240.

XIANG Yi-He. Discovery of Function and Structure of Deoxyribonucleic Acid Polymerase Ⅲ Holoenzyme[J]. Chinese Journal of Nature, 2012, 34(4): 231-240.