关键词: DNA聚合酶I, 活性中心模型, Klenow片段, 晶体结构, 右手模型

Abstract:

The process of finding the function and the structure of DNA polymerase is introduced. The key events include the proposition of active center model of the polymerase, the determination of molecular weight of the polymerase, the dissociation of the polymerase and achievement of active fragment, the determination of crystal structure of Klenow fragment binding DNA and of bacteriophage T7DNA replication. Based on the determined crystal structure, a right hand model was proposed.