自然杂志 ›› 2021, Vol. 43 ›› Issue (1): 32-38.doi: 10.3969/j.issn.0253-9608.2021.01.005
张冰洁①②,水雯箐①②
收稿日期:
2020-10-01
出版日期:
2021-02-25
发布日期:
2021-02-25
通讯作者:
水雯箐,通信作者,研究方向:组学与化学生物学。
ZHANG Bingjie①②, SHUI Wenqing①②
Received:
2020-10-01
Online:
2021-02-25
Published:
2021-02-25
摘要: 基于靶蛋白 配体亲合作用检测的亲和质谱技术与常规高通量筛选技术相辅相成,已发展成为药物先导化合物早期发 现流程中的关键技术之一。G蛋白偶联受体(GPCR)家族由于其特殊的生化性质,对基于亲合作用的配体筛选和配体表征 提出了巨大的技术挑战。文章主要介绍亲和质谱技术用于GPCR配体筛选的基本原理及其特点,并总结该技术用于大规模化 合物库及天然草本粗提物筛选的前沿进展。
张冰洁, 水雯箐. 发展亲和质谱技术加速G蛋白偶联受体的配体筛选和药物发现[J]. 自然杂志, 2021, 43(1): 32-38.
ZHANG Bingjie, SHUI Wenqing. Developing the affinity mass spectrometry technology for the discovery of chemical ligands and drug leads towards GPCR targets[J]. Chinese Journal of Nature, 2021, 43(1): 32-38.
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